Doctor of Philosophy, Harvard University (2010)
Kang Shen, Postdoctoral Faculty Sponsor
Morphometric studies in multicellular organisms are generally performed manually because of the complexity of multidimensional features and lack of appropriate tools for handling these organisms. Here we present an integrated system that identifies and sorts Caenorhabditis elegans mutants with altered subcellular traits in real time without human intervention. We performed self-directed screens 100 times faster than manual screens and identified both genes and phenotypic classes involved in synapse formation.
View details for DOI 10.1038/NMETH.2141
View details for Web of Science ID 000309519300018
View details for PubMedID 22902935
Neurons develop mutually exclusive dendritic domains through self-avoidance and tiling mechanisms. Two recent studies establish that this process is dependent on the restriction of dendrites to a two-dimensional plane through interactions with the extracellular matrix.
View details for DOI 10.1016/j.cub.2012.02.017
View details for Web of Science ID 000301890900010
View details for PubMedID 22440803